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Original Research Article | OPEN ACCESS

Studies on the expression and biological functions of ZIC5 in hepatocellular carcinoma

Pingpo Ming1, Weixing Wang2 , Chunyan Li3, Yongfa Zheng1, Wei Ge1

1Department of Oncology; 2Department of General Surgery; 3Department of Obstetrics, Renmin Hospital of Wuhan University, Wuhan City, Hubei Province 430060, China.

For correspondence:-  Weixing Wang   Email: wxwang999@163.com   Tel:+862788041911

Accepted: 29 October 2020        Published: 30 November 2020

Citation: Ming P, Wang W, Li C, Zheng Y, Ge W. Studies on the expression and biological functions of ZIC5 in hepatocellular carcinoma. Trop J Pharm Res 2020; 19(11):2295-2302 doi: 10.4314/tjpr.v19i11.7

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the expression of zinc finger protein of the cerebellum 5 (ZIC5) and its biological functions in hepatocellular carcinoma (HCC).
Methods: Sixty-five patients undergoing HCC surgery were selected. expression of ZIC5 in HCC and para-carcinoma tissue was examined by quantitative real time-polymerase chain reaction (qRT-PCR) and western blotting. The relationship between ZIC5 expression and clinicopathological features, postoperative survival rate, and prognosis of liver cancer patients was analyzed by t-test, Kaplan-Meier method, and Cox regression analysis, respectively. The effects of ZIC5 silencing on Huh-7 cell proliferation, migration, invasion, and apoptosis were assessed using Cell Counting Kit-8 (CCK-8), wound healing assay, Transwell assay, and flow cytometry, respectively.
Results: ZIC5 expression in liver cancer tissue was significantly higher than in the para-carcinoma tissue and was significantly correlated with TNM stage and differentiation degree (p < 0.001). The overall survival rate of patients with high ZIC5 expression level was significantly lower than that of patients with low ZIC5 expression (p < 0.01). ZIC5 expression, TNM stage, and differentiation degree were independent prognostic factors. ZIC5 silencing significantly inhibited the proliferative, migratory, invasive, and anti-apoptotic capacity of Huh-7 cells (p < 0.01).
Conclusion: ZIC5 is highly expressed in HCC, and this can promote liver cancer cell proliferation, migration, and invasion.

Keywords: Hepatocellular carcinoma, Cerebellar zinc finger structure 5, Prognosis, Migration, Proliferation

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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